Breakthrough News: A novel Separation-Sensing (SepSen) membrane has been reproted in Angew. Chem. Int. Ed.

Updated:7/14/2020   By:wtl

Jin’s group recently proposed a novel SepSen membrane performing synchronous blood sieving and serum analysis, which published a new paper “A novel separation-sensing membrane performing precise real-time serum analysis during blood drawing” in Angew. Chem. Int. Ed. (https://onlinelibrary.wiley.com/doi/10.1002/anie.202008241).

This membrane was constructed by a heterogeneous-nanostructured architecture, wherein a surface nanoporous layer continuously extracts serum, while the biosensing nanochannels underneath dynamically recognise biotargets, thereby achieving a continuous testing of vital clinical indices as blood is drawn. By precisely controlling the pore structure and nanoshape of biosensing crystals, this membrane achieved accurate and online glucose and lactate monitoring in patients with a variety of medical conditions within 1 min, which is one order of magnitude faster than the state-of-the-art techniques. Moreover, various kinds of bio-recognisers can be introduced into this membrane to accurately detect glutamate, transaminase, and cancer biomarkers.

This work presents a new research direction that combining membrane science and electrochemical analysis together, which is promising to obviously decrease the risk of clinical treatment during surgery and emergency.

血液检验是疾病诊断最主要的手段之一,许多突发性死亡均是由特定组分浓度的急剧改变所造成。在临床手术中,呼吸、心跳、血氧等指标均已实现了在线监控,然而,由于血液的凝血特性与易溶血性,现有检验技术难以在线跟踪血液关键指标的动态波动。针对以上难题,今日,南京工业大学的金万勤团队设计了一种具有梯度纳米结构的分离传感膜(SepSen),并联合南京大学医学院附属鼓楼医院的马正良团队,成功实现临床术中多种血液指标的动态精准监控。

在血液检验中,血液的自凝性易造成测试元件的污染,同时红细胞离体后细胞壁因渗透压改变极易破损,造成检测结果的偏差。因此,现有临床血液检测技术大多需要提前对全血离心分离或以一次性试纸模式实现特定组分的定量检测。然而,以上技术均为非在线式且仅能采集离散样本,无法反映一段时间内患者体内血组分的动态波动。

南京工业大学团队利用限域纳米调控技术,在陶瓷中空纤维表面与孔道内同步梯度生长兼具成膜性及电催化活性的普鲁士蓝/聚吡咯双功能膜层。在外表面,由于反应动力学速率较大,材料的晶间交互生长行为起主导作用,形成具有多孔结构的血液分离膜层;而孔道内,晶体生长被限定在200nm的受限空间内,其结晶速率降低,趋向于形成规整立方结构晶体,形成高电催化活性的传感膜层。当血液连续抽取时,分离膜层可动态无损截留血细胞及纤维蛋白原,仅让血清进入孔道内;血清中的特定组分在酶、DNA或抗体的识别下产生电信号,并经由孔内纳米立方材料的电催化放大后输出。该分离传感膜可将传统血检中抽血、分离、分析等过程集为一体,时间由30分钟以上缩短为1分钟,实现对血糖、乳酸、谷氨酸、转氨酶及癌标物的高灵敏检测。同时,所开发分离传感膜微型器件,实现了在多种临床手术中血糖及血乳酸的精准动态监测,表现出优异的长时间稳定性。该技术可为临床手术及急救中构建关键血液指标的新型动态监控平台,为膜科学与临床医学的交叉研究提供新的思路。